Search results for "SYNOVIAL TISSUE"

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Extinct type of human parvovirus B19 persists in tonsillar B cells

2017

Parvovirus B19 (B19V) DNA persists lifelong in human tissues, but the cell type harbouring it remains unclear. We here explore B19V DNA distribution in B, T and monocyte cell lineages of recently excised tonsillar tissues from 77 individuals with an age range of 2–69 years. We show that B19V DNA is most frequent and abundant among B cells, and within them we find a B19V genotype that vanished from circulation >40 years ago. Since re-infection or re-activation are unlikely with this virus type, this finding supports the maintenance of pathogen-specific humoral immune responses as a consequence of B-cell long-term survival rather than continuous replenishment of the memory pool. Moreover, we …

0301 basic medicineSYNOVIAL TISSUEvirusesPalatine TonsilGeneral Physics and AstronomyAntibodies ViralGenotypeINFECTIONParvovirus B19 HumanREAL-TIME PCRChildCells CulturedB-LymphocytesMultidisciplinarybiologyQcell type harbouringvirus diseasesU937 CellsMiddle Aged3. Good healthHUMAN ERYTHROVIRUSESsolutReal-time polymerase chain reactionmedicine.anatomical_structurePLASMA-CELLSChild PreschoolGENETIC DIVERSITYAntibodyAdultCell typeAdolescentGenotypeBONE-MARROWScience030106 microbiologyQUANTITATIVE PCRta3111ArticleGeneral Biochemistry Genetics and Molecular BiologyCell LineParvoviridae InfectionsYoung Adult03 medical and health sciencesImmune systemCell Line TumormedicineHumansAgedB cellsparvovirus B19ParvovirusMonocyteta1182General ChemistryDNAvirus typesbiology.organism_classificationVirologyCELLULAR CORECEPTOR030104 developmental biologyCell cultureDNA ViralImmunologybiology.proteincells3111 BiomedicineNature Communications
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IL-9 IN PsA

2016

Objective. To investigate the expression and tis- sue distribution of Th9-related cytokines in patients with psoriatic arthritis (PsA). Methods. Quantitative gene expression analysis of Th1, Th17, and Th9 cytokines was performed in intestinal biopsy samples obtained from patients with PsA, HLA2B272positive patients with ankylosing spondylitis (AS), patients with Crohn’s disease (CD), and healthy controls. Expression and tissue distribu- tion of interleukin-23 (IL-23), IL-17, IL-22, IL-9, and IL-9 receptor (IL-9R) were evaluated by immunohisto- chemistry and confocal microscopy. Flow cytometry was used to study the frequency of Th9 cells among periph- eral blood, lamina propria, and synovial…

InflammationMalePsoriatic arthritis gut inflammation synoviasynoviaArthritis PsoriaticSynovial MembranePsoriatic ArthritisInterleukin-9T-Lymphocytes Helper-InducerReceptors Tumor Necrosis FactorIntestinesSettore MED/16 - ReumatologiaGene Expression RegulationTh9 cellHumansFemaleUstekinumabGutSynovial Tissuegut inflammationInterleukin-9 Th9 cells Gut Synovial Tissue Psoriatic Arthritis
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